F3393
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 | Immunohistochemistry Immunoperoxidase staining (PAP) of a freshly explanted gliobastoma multiforme (WHO grade IV, SAMS grade 4) in culture (passage # 3) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). Intracytoplasmatically, homogeneous, high bFGF expression is shown within highly polymorphic tumor cells. The tumor cells on glass coverslips were methanol/acetone (100%, ice-cold, 1:1) fixed without any enzymatic or antigen-demasking pre-processing. From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany. |
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F3393
Sigma
Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit
fractionated antiserum, lyophilized powder
| Synonym: | Anti-bFGF, Anti-FGF2 |
| MDL number: | MFCD01322020 |
Description
| Immunogen | synthetic peptide sequence corresponding to bovine FGF2 (1-24). |
| Physical form | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. |
Properties
| antibody form | fractionated antiserum |
| clone | (polyclonal) |
| form | lyophilized powder |
| species reactivity | bovine, human, rat |
| application(s) | immunoblotting: 1:2,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| total impurities | Endotoxin, tested |
| Gene Information | human ... FGF2(2247) |
| rat ... Fgf2(54250) | |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
Related Products
| Related product | F5537, Anti-Fibroblast Growth Factor-Basic antibody produced in rabbit |
| F5305, Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse | |
| F5180, Anti-Fibroblast Growth Factor-Basic antibody produced in goat | |
| F6162, Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse |
References
| reference | Stan, A.C., et al. J. Neurosurg. 82, 1044, (1995) Abstract |
| Gloe, T., et al., Shear stress-induced release of basic fibroblast growth factor from endothelial cells is mediated by matrix interaction via integrin α(v)β3. J. Biol. Chem. 277, 23453-23458, (2002) Abstract | |
| Belluardo, N., et al., Nicotine-induced fibroblast growth factor-2 restores the age-related decline of precursor cell proliferation in the subventricular zone of rat brain. Brain Res. 1193, 12-24, (2008) Abstract | |
| Alonso, M., et al., FGF2 plays a key role in embryonic cerebrospinal fluid trophic properties over chick embryo neuroepithelial stem cells. Dev. Biol. 297, 402-416, (2006) Abstract |
